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商品详细Cusabio/SARS-CoV-2n抗体对2/1对/CSB-EAP33255A2
Cusabio/SARS-CoV-2n抗体对2/1对/CSB-EAP33255A2
Cusabio/SARS-CoV-2n抗体对2/1对/CSB-EAP33255A2
商品编号: CSB-EAP33255A2
品牌: Cusabio
市场价: ¥20000.00
美元价: 10000.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

SARS-CoV-2 N Antibody Pair 2

Datasheet
CodeCSB-EAP33255A2
SizeUS$1000
Image
  • CSB-EAP33255A2 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells. The SARS-CoV-2 Nucleoprotein (N) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Nucleoprotein (N) is added to detect the captured SARS-CoV-2 Nucleoprotein (N) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
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Product Details

Uniprot No. P0DTC9
Alternative NamesNucleocapsid protein, NC, protein N, N
Species ReactivityHuman Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
ImmunogenRecombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
Immunogen SpeciesHuman Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
ConcentrationIt differs from different batches. Please contact us to confirm it.
FormLiquid
Protein NamesHuman Novel Coronavirus Nucleoprotein (N)
HostCapture: MouseDetection: Mouse
BufferCapture: 50% Glycerol, 0.01M PBS, PH 7.4Detection: 50% Glycerol, 0.01M PBS, PH 7.4
ComponentsCapture: CSB-EAP33255A2CDetection: CSB-EAP33255A2B(HRP)Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
Tested ApplicationsS-ELISA
Troubleshooting and FAQsAntibody FAQs
NotesWe recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml. Optimal dilutions should be determined experimentally by the researcher.
Lead TimeBasically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

FunctionNucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.
Subcellular LocationVirion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus
Protein FamiliesBetacoronavirus nucleocapsid protein family
品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。