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商品详细Cusabio/SARS-CoV-2N ELISA试剂盒/96T,5×96T,10×96T/CSB-EL33251
Cusabio/SARS-CoV-2N ELISA试剂盒/96T,5×96T,10×96T/CSB-EL33251
Cusabio/SARS-CoV-2N ELISA试剂盒/96T,5×96T,10×96T/CSB-EL33251
商品编号: CSB-EL33251
品牌: Cusabio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

SARS-CoV-2 N ELISA Kit

Instructions
CodeCSB-EL33251
Size96T,5×96T,10×96T
Trial Size24T ELISA kits trial application
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Product Details

Target NameNovel Coronavirus Nucleoprotein (SARS-CoV-2 N)
Alternative Names2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleoprotein
AbbreviationSARS-CoV-2 N
Uniprot No.P0DTC9
Sample Typesserum, plasma, swabs
Detection Range3.12 ng/mL-200 ng/mL
Sensitivity0.78 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaInfectious Diseases
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean(ng/ml)25.3924.4626.3126.6526.3124.19
SD0.0270.0250.0310.0410.0350.037
CV(%)5.75.466.338.267.138.01
Typical DataThese standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2AverageCorrected
2003.2283.1043.1663.032
1002.0452.1692.1071.973
501.0010.9310.9660.832
250.4690.5070.4880.354
12.50.2680.2760.2720.138
6.250.1960.2040.2000.066
3.120.1680.1610.1650.031
00.1360.1320.134 
Troubleshooting and FAQsELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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Target Data

FunctionNucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.
Subcellular LocationVirion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus
Protein FamiliesBetacoronavirus nucleocapsid protein family
品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。