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His-Tag Monoclonal Antibody
| Code | CSB-MA000159 |
| Size | US$167 |
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Product Details
| Description | His-Tag monoclonal antibody CSB-MA000159 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The immunogen is a short peptide. It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.This His-Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. An efficient detection and purification system based on fusion proteins can be established using the His tag. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted. | ||||||||
| Target Names | His-Tag | ||||||||
| Raised in | Mouse | ||||||||
| Species Reactivity | N/A | ||||||||
| Immunogen | Synthetic Peptide | ||||||||
| Conjugate | Non-conjugated | ||||||||
| Isotype | IgG | ||||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||
| Buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||||||||
| Form | Liquid | ||||||||
| Tested Applications | ELISA, WB, IF, IP | ||||||||
| Recommended Dilution |
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| Protocols | ELISA ProtocolWestern Blotting(WB) ProtocolImmunofluorescence (IF) ProtocolImmunoprecipitation (IP) Protocol | ||||||||
| Troubleshooting and FAQs | Antibody FAQs | ||||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Citations
 | Somatic SF3B1 hotspot mutation in prolactinomas. C Li,Nature Communications,2020 Applications: GST pull-downAntibody dilution factor: 1:200Review: We examined Pit-1 protein binding in relation to ESRRG alternative splicing status. Pit-1 protein fused to glutathione S-transferase (GST) pull-down HIS-tagged cryptic ESRRG, whereas it bound weakly to canonical ESRRG PMID: 32427851 |
 | When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell. Siqueira RAGB, et al,Toxicon,2019 Applications: ELISAAntibody dilution factor: 1: 4000Review: Murine B16F10 and L929 cell lines (2× 10 5 ) were fixed on plates and then blocked with 2% BSA solution. After that, Rechistatin, Echistatin, LgRec1 or EGFP (1; 3; 6 and 9μM) were added to the plates followed by incubation for 2 h at 37°C. The binding of the recombinant proteins to the cells was assessed using anti-His (Cusabio®) mAb followed by anti-mouse IgG-peroxidase conjugate (Sigma® ). The reaction was measured by spectrophotometer at 492 nm, the higher the absorbance the grater the binding of the toxins to the cells. A) Binding of recombinant toxins on murine B16F10 melanoma cells; B) Binding of recombinant toxins on murine L929 cells. The results were expressed as mean ± SD (n = 3), ***p < 0.001;="">**p < 0.01="" and="">*p < 0.05="" comparing="" rechistatin,="" echistatin,="" lgrec1="" and="" egfp="" groups="" com-="" pared="" with="">PMID: 31251993 |
 | Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus. Huang D, et al,Nature Plants,2018 Application: Pull-down assayReview: Competitive binding assays of CgRuby1 and CgRuby2Short binding to CgbHLH1. The mixture of HIS-CgRuby1 and FLAG CgRuby2Short was added to immobilized GSTCgbHLH1. The precipitates were detected using western blot analysis with anti-HIS, anti-FLAG or anti-GST antibodies. The gradient indicates the increasing amount of FLAG-CgRuby2Short. These experiments were repeated independently twice with similar results.PMID: 30374094 |
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