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当前位置: 首页 > 产品中心 > Internal_Control_Antibody > Cusabio/Myc tag Monoclonal Antibody/100μg/CSB-MA000041M0m
商品详细Cusabio/Myc tag Monoclonal Antibody/100μg/CSB-MA000041M0m
Cusabio/Myc tag Monoclonal Antibody/100μg/CSB-MA000041M0m
Cusabio/Myc tag Monoclonal Antibody/100μg/CSB-MA000041M0m
商品编号: CSB-MA000041M0m
品牌: Cusabio
市场价: ¥4000.00
美元价: 2000.00
产地: 美国(厂家直采)
公司:
产品分类: 内参抗体
公司分类: Internal_Control_Antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Myc tag Monoclonal Antibody

Datasheet
CodeCSB-MA000041M0m
SizeUS$200Purchase it in Cusabio online store(only available for customers from the US)
Image
  • WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/mlLane 1: Recombinant Myc-tagged fusion protein at 50ngLane 2: Recombinant Myc-tagged fusion protein at 25ngLane 3: Recombinant Myc-tagged fusion protein at 12.25ngLane 4: Recombinant Myc-tagged fusion protein at 6.25ngLane 5: Recombinant Myc-tagged fusion protein at 3.125ngLane 6: Recombinant Myc-tagged fusion protein at 1.5625ngSecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 50 kdObserved band size: 50 kd

  • Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating MYC-tag with transfected HEK293 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)Lane 3: Transfected HEK293 whole cell lysate (10µg)

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Product Details

Description
Myc tag monoclonal antibody CSB-MA000041M0m was produced in the mouse immunized by using the EQKLISEEDL (Myc) synthetic peptide conjugate to KLH as the immunogen. The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag. This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.This Myc tag monoclonal antibody was tested in the ELISA, WB, IF and IP. The non-conjugated IgG1 got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like. It doesn’t have species restricted.
Target NamesMyc tag
Alternative NamesbHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
Raised inMouse
Species ReactivityN/A
ImmunogenEQKLISEEDL (Myc) synthetic peptide conjugate to KLH
ConjugateNon-conjugated
ClonalityMonoclonal
IsotypeIgG1
Clone No.5F92F9
Purification Method>95%, Protein G purified
ConcentrationIt differs from different batches. Please contact us to confirm it.
BufferPreservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
FormLiquid
Tested ApplicationsELISA, WB, IF, IP
Recommended Dilution
ApplicationRecommended Dilution
WB1:500-1:5000
IF1:100-1:300
IP1:1000-1:1500
ProtocolsELISA ProtocolWestern Blotting(WB) ProtocolImmunofluorescence (IF) ProtocolImmunoprecipitation (IP) Protocol
Troubleshooting and FAQsAntibody FAQs
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead TimeBasically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex. Abad M A, et al,The Journal of Cell Biology,2019

Sample type: HeLa Kyoto cells Sample species: MouseAntibody dilution factor: 1:1000Application: WBReview: Analysis of the expression levels of the Myc-Borealin constructs used in Fig. 4 (B and C).PMID: 31570499

Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Jeon Y.et al,Plant Mol Biol,2017

Application: Immunoblotting Antibody dilution factor: 1:5000Review: Since YFPN and YFPC are fused to Myc and HA tags, respectively, NbRbgA:YFPN and cpRPL:YFPC proteins were detected by immunoblotting using anti-Myc and anti-HA antibodies, respectively.PMID: 29038916

Customer Reviews and Q&A

Customer Reviews
Average Rating:
5.0 -2 reviews

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Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Applications : Western Blot (WB)

Sample type: Cells

Sample dilution: 1:500

Review: Antibody specificity is very good.

By Anonymous

Applications : Western Blot (WB)

Sample type: HEK293 cell lysate

Sample dilution: 1:1000

Review: Myc antibody has good specificity.

By Anonymous

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品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。