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当前位置: 首页 > 产品中心 > antibody > Cusabio/GFP Monoclonal Antibody/100μg/CSB-MA000051M0m
商品详细Cusabio/GFP Monoclonal Antibody/100μg/CSB-MA000051M0m
Cusabio/GFP Monoclonal Antibody/100μg/CSB-MA000051M0m
Cusabio/GFP Monoclonal Antibody/100μg/CSB-MA000051M0m
商品编号: CSB-MA000051M0m
品牌: Cusabio
市场价: ¥4000.00
美元价: 2000.00
产地: 美国(厂家直采)
公司:
产品分类: 抗体
公司分类: antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

GFP Monoclonal Antibody

Datasheet
CodeCSB-MA000051M0m
SizeUS$200Purchase it in Cusabio online store(only available for customers from the US)
Image
  • WB: Mouse Anti-GFP monoclonal antibody at 0.1μg/mlLine 1:HEK-293 cell lysateLine 2:GFP transfected HEK-293 cell lysateSecondaryGoat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size : 30kdObserved band size : 30kd

  • IP:Result of anti-GFP-tag Monoclonal antibodyLine 1:Control IgGLine 2:PrecipitateingGFP-tagged fusion proteinLine 3:GFP Transfected HEK-293 cells lysateSecondaryGoat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size : 30kdObserved band size : 30kd

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Product Details

Description
GFP monoclonal antibody CSB-MA000051M0m was produced in the mouse immunized by using the Recombinant GFP Protein as the immunogen. The target protein GFP is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.This GFP Monoclonal Antibody was tested in the ELISA, WB and IP. The non-conjugated IgG2b got purified by protein G and reached up to 95% in purity. Anti-GFP antibodies provide a convenient method for visualizing GFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. It doesn’t have species restricted.
Target NamesGFP
Alternative NamesCFP, eGFP, eYFP, GFP, GFP tag, YFP
Raised inmouse
Species ReactivityN/A
ImmunogenRecombinant GFP Protein
ConjugateNon-conjugated
Clonalitymonoclonal
IsotypeIgG2b
Purification Method>95%,Protein G purified
ConcentrationIt differs from different batches. Please contact us to confirm it.
BufferPreservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Formliquid
Tested ApplicationsELISA,WB,IP
Recommended Dilution
ApplicationRecommended Dilution
WB1:500-1:5000
IP1:200-1:2000
ProtocolsELISA ProtocolWestern Blotting(WB) ProtocolImmunoprecipitation (IP) Protocol
Troubleshooting and FAQsAntibody FAQs
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead TimeBasically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Porcine DNAJB6 promotes PCV2 replicationvia enhancing the formation of autophagyin host cells. C Han,Vet Res,2020

Applications: IPReview: PCV2 Cap interacts with pDNAJB6 in transfected cells. HEK293T cells were co-transfected with GFP-Cap and Flag-pDNAJB6 expression plasmids or co-transfected with the pEGFP-N1 control vector and the Flag-pDNAJB6 expression plasmid. The cells transfected with pEGFP-N1 control vector, GFP-Cap expression plasmid or Flag-pDNAJB6 expression plasmid alone served as controls. The interaction of GFP-Cap with Flag-pDNAJB6 was identified through immunoprecipitation using anti-Flag antibodies or anti-GFP antibodies.PMID: 32381067

The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. Gomez-H L, et al,Plos Genetics,2019

Applications: IPAntibody dilution factor: 1: 1000Review:HEK293T cells were transfected with Flag-PSMA8 and GFP-SYCP1. Protein complexes were immunoprecipitated overnight with either an anti-Flag or anti-EGFP or IgGs (negative control), and were analyzed by immunoblotting with the indicated antibody. PSMA8 co-immunoprecipitates with SYCP1.

Microscopic analysis of colonization of Colletotrichum abscissum in citrus tissues. Daiani CristinaSavi,microbiological research,2019

Applications: IHCAntibody dilution factor: 1: 500Review:fluorescent microscopic analysis 12 h after inoculation showing the fungal hyphae expressing GFP (in red) penetrating the floral tissue (in blue).PMID: 31284941

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品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。