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商品详细Cusabio/GAPDH单克隆抗体/100μl/CSB-MA000071M

Cusabio/GAPDH单克隆抗体/100μl/CSB-MA000071M

商品编号： CSB-MA000071M0m

品牌： Cusabio

市场价： ¥4000.00

美元价： 2000.00

产地：美国(厂家直采)

公司：

产品分类：重组抗体

公司分类： Recombinant_antibodies

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

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GAPDH Monoclonal Antibody

GAPDH Monoclonal Antibody

Datasheet

Code	CSB-MA000071M0m
Size	US$200Purchase it in Cusabio online store(only available for customers from the US)
Image	Western BlotPositive WB detected in: 15μg hela whole cell lysateGAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min Western BlotPositive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μgAll lanes: GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All lanes: GAPDH antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 30s Western BlotPositive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysateAll lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 30s Western BlotPositive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissue All lanes GAPDH antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 1min Western BlotPositive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissueAll lanes GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of HepG2 cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000071M0m in Hela whole cell lysate.Lane 2: CSB-MA000071M0m (1μl) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg) For western blotting, the blot was detected with CSB-MA000071M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 Overlay histogram showing Hela cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed. Overlay histogram showing Jurkat cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
Citations
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Customer Reviews and Q&A
Target Data

Product Details

Full Product Name

Mouse anti-Human GAPDH Monoclonal antibody

Uniprot No.

P04406

Target Names

GAPDH

Alternative Names

GAPDH; G3PD; GAPD; MGC88685

Raised in

Mouse

Species Reactivity

Human, Rat, Rabbit

Immunogen

Recombinant Human GAPDH protein (3-335AA)

Immunogen Species

Human

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

14C2F11

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:5000-1:1600000
IHC	1:50-1:500
IF	1:50-1:200
IP	1µl-2µl
FC	1:100-1:300

Protocols

ELISA ProtocolWestern Blotting(WB) ProtocolImmunohistochemistry (IHC) ProtocolImmunoprecipitation (IP) ProtocolImmunofluorescence (IF) ProtocolFlow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

	Concurrent EPA and DHA Supplementation Impairs Brown Adipogenesis of C2C12 Cells. S Ghnaimawi,Frontiers in Genetics,2020 Applications: Western BlotAntibody dilution factor: 1:1000Review: Protein level measurement by western blot of myogenesis regulating genes in C2C12 and GAPDH as housekeeping gene n = 4.PMID: 32595696
	Quadrella incana (Capparaceae) Leaf Extract Enhances Proliferation and Maintenance of Neural Stem/Progenitor Cells through Upregulating Glycolytic Flux and Redox Potential. M Kang,Oxid Med Cell Longev,2020 Applications: Western blotAntibody dilution factor: 1:1,000Review: Western blot analysis of Bmi1, βIII tubulin, and phospho-Histone 3 (Ser 10) in NSPCs kept under proliferating media upon treatment for 3 days with DMSO, Quadrella incana leaf extract, or six diﬀerent plant crude extracts. GAPDH was used as a loading control. GAPDH was used as a loading control. PMID: 32411330
	Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane. B Lee.et al,Biochimica et biophysica acta-molecular cell research,2017 Applications: WBAntibody dilution factor: 1: 5000Review: HeLa cells were treated with si BAF #2 or si Control for 72 h. Cells were fractionated and subjected to western blot analyses (upper panel). Fractionation was verified by using Lamin A/C antibody (nuclear fraction) or GAPDH antibody (cytosolic fraction). The intensity of the cytosolic emerin was analyzed using ImageJ software (lower panel). PMID: 27865926
	A fluorescence method for determination of glucose transport by intestinal BBMV of common carp. Yang LP.et al,Anal Biochem,2017 Application: Immunoblot analysesReview: Immunoblot analyses show the presence of SGLT-1 on BBMV of common carp.PMID: 28847591

Customer Reviews and Q&A

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