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商品详细Cusabio/E-Tag Monoclonal Antibody/100μl/CSB-MA000151M0m

Cusabio/E-Tag Monoclonal Antibody/100μl/CSB-MA000151M0m

商品编号： CSB-MA000151M0m

品牌： Cusabio

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：抗体套装

公司分类： Antibody_set

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

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E-Tag Monoclonal Antibody

E-Tag Monoclonal Antibody

Datasheet

Code	CSB-MA000151M0m
Size
Image	Western BlotPositive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ngAll lanes: E-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 36 kDaObserved band size: 36 kDa Western BlotPositive WB detected in: E-tagged fusion proteinAll lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 36 kDaObserved band size: 36 kDa Immunoprecipitating E-Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)Lane 3: 293F transfected whole cell lysate (20µg) ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)Antigen at dilution of 1µg/mlE-Tag antibody at (0-0.15µg)Goat polyclonal to Mouse IgG at 1/50000 dilution Western BlotPositive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-TagAll lanes: E-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 30, 68, 55, 67-157kDaObserved band size: 30, 68, 55, 67-157kDa
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Product Details
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Product Details

Target Names

E-Tag

Alternative Names

E-Tag

Raised in

Mouse

Species Reactivity

All

Immunogen

GAPVPYPDPLEPRC synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Clonality

Monoclonal Antibody

Isotype

IgG1

Clone No.

11H12B3

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
ELISA	1:4000-1:8000
WB	1:1000-1:320000
IP	1μg-5μg

Protocols

ELISA ProtocolWestern Blotting(WB) ProtocolImmunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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品牌介绍

CUSABIO是一家集研发，生产和销售于一体的国家高科技企业。经过12年的努力，CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症，细胞生物学，免疫学，神经科学，表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体，7000 +种重组蛋白，603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。

公司介绍

品牌分类

小分子; Molecular Biology Product 活性蛋白质 n. 衣物和装备;成套用品;配套元件; n. 抗体; n. [化]朊，蛋白（质）;

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官网：http://www.cusabio.com/