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当前位置: 首页 > 产品中心 > Tissue_staining_apparatus > Cusabio/Pfu DNA聚合酶/1000单位/CSB004B
商品详细Cusabio/Pfu DNA聚合酶/1000单位/CSB004B
Cusabio/Pfu DNA聚合酶/1000单位/CSB004B
Cusabio/Pfu DNA聚合酶/1000单位/CSB004B
商品编号: CSB004B
品牌: Cusabio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 组织染色机
公司分类: Tissue_staining_apparatus
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  • Specification

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CodeCSB004B
Size1000 Units
RelevancePfu DNA Polymerase is a recombinant 90kDa DNA polymerase, originally inducibly expressed in and purified from Pyrococcus furiosis that containing DNA polymerase, thermostable gene. It has the same function as native Pfu DNA polymerase. Pfu Taq DNA Polymerase has a 5 ' -3 ' DNA polymerase activity and 3 ' -5 ' exonuclease activity and can correct the base mismatch during PCR process. Elongation rate is 0.5-1kb/min. It does not exhibit nucleotidyl terminal transferase activity so its amplification products can be directly used for cloning in blunt-ended vectors.
Intended Use1, DNA amplification by Polyermerase Chain Reaction (PCR);2, High-fidelity DNA amplification, cloning and expression;3, DNA sequencing;4, Site directed mutagenesis.
Quality ControlThe purity of enzyme is above 97%, evaluated by SDS-PAGE. It’s validated to be no exogenous nucleic acid enzymatic activity. There is no residual host cell DNA by PCR detection. Functionally tested in amplification of a single-copy gene from human genomic DNA. No obvious enzyme activity changes observed after storing at room temperature for one week.
Storage-20°C
Length/Coupling Ratio5U/μl
Component
Pfu DNA Polymerase400ul
10×Pfu buffer (Mg2+Plus)1ml×4
dNTP Mixture (10mM each)100μl×4
6×loading buffer1ml×4
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品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。