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商品详细Cusabio/重组Sindbis病毒结构多蛋白,部分/100μg/CSB-CF361018SHZb2
Cusabio/重组Sindbis病毒结构多蛋白,部分/100μg/CSB-CF361018SHZb2
Cusabio/重组Sindbis病毒结构多蛋白,部分/100μg/CSB-CF361018SHZb2
商品编号: CSB-CF361018SHZb2
品牌: Cusabio
市场价: ¥72120.00
美元价: 36060.00
产地: 美国(厂家直采)
公司:
产品分类: 血清纯化蛋白
公司分类: Serum_purified_protein
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Recombinant Sindbis virus Structural polyprotein,partial

CodeCSB-CF361018SHZb2
SizeUS$3606
Image
  • SDS-PAGE - Recombinant Sindbis virus Structural polyprotein

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

PurityGreater than 85% as determined by SDS-PAGE.
Uniprot No.P03316
Research Areaothers
Alternative NamesStructural polyprotein; p130) [Cleaved into: Capsid protein; EC 3.4.21.90; Coat protein; C); Precursor of protein E3/E2; p62; pE2); Assembly protein E3; Spike glycoprotein E2; E2 envelope glycoprotein); 6K protein; Spike glycoprotein E1; E1 envelope glycoprotein)]
SpeciesSindbis virus (SINV)
Sourcein vitro E.coli expression system
Expression Region807-1245aa
Target Protein SequenceYEHATTVPNVPQIPYKALVERAGYAPLNLEITVMSSEVLPSTNQEYITCKFTTVVPSPKIKCCGSLECQPAAHADYTCKVFGGVYPFMWGGAQCFCDSENSQMSEAYVELSADCASDHAQAIKVHTAAMKVGLRIVYGNTTSFLDVYVNGVTPGTSKDLKVIAGPISASFTPFDHKVVIHRGLVYNYDFPEYGAMKPGAFGDIQATSLTSKDLIASTDIRLLKPSAKNVHVPYTQASSGFEMWKNNSGRPLQETAPFGCKIAVNPLRAVDCSYGNIPISIDIPNAAFIRTSDAPLVSTVKCEVSECTYSADFGGMATLQYVSDREGQCPVHSHSSTATLQESTVHVLEKGAVTVHFSTASPQANFIVSLCGKKTTCNAECKPPADHIVSTPHKNDQEFQAAISKTSWSWLFALFGGASSLLIIGLMIFACSMMLTSTRRNote: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight65.9kDa
Protein LengthPartial
Tag InfoN-terminal 10xHis-SUMO-tagged
FormLiquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.Note: If you have any special requirement for the glycerol content, please remark when you place the order.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQsProtein FAQs
Storage ConditionStore at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf LifeThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead TimeDelivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
NotesRepeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

FunctionCapsid protein
Gene References into Functions
  1. The authors conclude that H230 of E1 protein is essential for the assembly of complete infectious Sindbis virus virions and that the presence of an amino acid at E2 position 209 is required for complete budding of Sindbis virus particles although several different amino acids can be at this location without affecting the titer. PMID: 27412592
  2. Mutating conserved cysteines in the e2 glycoprotein causes virus-specific assembly defects. PMID: 22238319
  3. Interactions of the cytoplasmic domain of E2 with nucleocapsid cores promote alphavirus budding. PMID: 22190727
  4. analysis of molecular links between the E2 envelope glycoprotein and nucleocapsid core in Sindbis virus PMID: 22001018
  5. Interaction of E2 glycoprotein with heparan sulfate is crucial for cellular infection of Sindbis virus PMID: 20300181
  6. Xray structure of spike, representing an intermediate in fusion process and clarifying maturation process; trimer of E2-E1 is similar to spikes in the neutral pH virus; amino- and carboxy-terminal domains of E2 form immunoglobulin-like folds PMID: 21124457
  7. specific amino acids within the region comprising amino acids 81 to 113 responsible for the encapsidation of the genomic RNA have been identified. PMID: 18305029
  8. The data indicate that the role of the cysteine residues in E3 is not primarily structural: it is hypothesized that E3 has an enzymatic or functional role in virus assembly, and these possibilities are further discussed. PMID: 19109378

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Subcellular LocationCapsid protein: Virion, Host cytoplasm, Host cell membrane, SUBCELLULAR LOCATION: Precursor of protein E2: Virion membrane, Single-pass type I membrane protein, Host cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Spike glycoprotein E2: Virion membrane, Single-pass type I membrane protein, Host cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Protein 6K: Host cell membrane, Multi-pass membrane protein, Virion membrane, Multi-pass membrane protein, SUBCELLULAR LOCATION: Spike glycoprotein E1: Virion membrane, Single-pass type I membrane protein, Host cell membrane, Single-pass type I membrane protein
Protein FamiliesAlphavirus structural polyprotein family
Database Links

KEGG: vg:1502155

品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。