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当前位置: 首页 > 产品中心 > leicabiosystems > Cusabio/Rat fibroblast growth factor 21 (FGF21) ELISA kit/96T,5×96T,10×96T/CSB-EL008627RA
商品详细Cusabio/Rat fibroblast growth factor 21 (FGF21) ELISA kit/96T,5×96T,10×96T/CSB-EL008627RA
Cusabio/Rat fibroblast growth factor 21 (FGF21) ELISA kit/96T,5×96T,10×96T/CSB-EL008627RA
Cusabio/Rat fibroblast growth factor 21 (FGF21) ELISA kit/96T,5×96T,10×96T/CSB-EL008627RA
商品编号: CSB-EL008627RA
品牌: Cusabio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 病理产品
公司分类: leicabiosystems
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Rat fibroblast growth factor 21 (FGF21) ELISA kit

Instructions
CodeCSB-EL008627RA
Size96T,5×96T,10×96T
Trial Size24T ELISA kits trial application
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Product Details

Target Namefibroblast growth factor 21
AbbreviationFGF21
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range23.5 pg/mL-1500 pg/mL
Sensitivity5.8 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaSignal Transduction
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %94-102
1:2Average %91
Range %86-97
1:4Average %106
Range %102-114
1:8Average %95
Range %90-100
Recovery
The recovery of rat FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)8580-90
EDTA plasma (n=4)9691-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
15002.646 2.772 2.709 2.590
7501.973 1.932 1.953 1.834
3751.217 1.302 1.260 1.141
187.50.789 0.793 0.791 0.672
940.463 0.449 0.456 0.337
470.302 0.281 0.292 0.173
23.50.195 0.205 0.200 0.081
00.121 0.117 0.119
Troubleshooting and FAQsELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations

  • Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy Y Gao,Redox Biology,2020

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品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。