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商品详细库萨比奥/马胰岛素样生长因子1(IGF1)ELISA试剂盒/96T,5×96T,10×96T/CSB-EL011086HO
库萨比奥/马胰岛素样生长因子1(IGF1)ELISA试剂盒/96T,5×96T,10×96T/CSB-EL011086HO
库萨比奥/马胰岛素样生长因子1(IGF1)ELISA试剂盒/96T,5×96T,10×96T/CSB-EL011086HO
商品编号: CSB-EL011086HO
品牌: Cusabio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 其它类仪器
公司分类: Other_instruments
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Horse insulin-like growth factor 1 (IGF1) ELISA kit

Instructions
CodeCSB-EL011086HO
Size96T,5×96T,10×96T
Trial Size24T ELISA kits trial application
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Product Details

Target Nameinsulin-like growth factor 1 (somatomedin C)
Alternative NamesIGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit; Fragment ELISA Kit
AbbreviationIGF1
Uniprot No.P51458
SpeciesEquus caballus (Horse)
Sample Typesserum, plasma, tissue homogenates
Detection Range12.5 ng/mL-800 ng/mL
Sensitivity3.12 ng/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaSignal Transduction
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %89-95
1:2Average %95
Range %90-104
1:4Average %92
Range %87-100
1:8Average %89
Range %80-97
Recovery
The recovery of horse IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9587-101
EDTA plasma (n=4)8782-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8001.961 1.944 1.953 1.820
4001.488 1.605 1.547 1.414
2001.178 1.213 1.196 1.063
1000.912 0.902 0.907 0.774
500.669 0.657 0.663 0.530
250.405 0.396 0.401 0.268
12.50.260 0.249 0.255 0.122
00.137 0.129 0.133
Troubleshooting and FAQsELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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Target Data

FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Subcellular LocationSecreted
Protein FamiliesInsulin family
Database Links

STRING: 9796.ENSECAP00000008158

UniGene: Eca.12985

品牌介绍
CUSABIO是一家集研发,生产和销售于一体的国家高科技企业。经过12年的努力,CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症,细胞生物学,免疫学,神经科学,表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体,7000 +种重组蛋白,603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。