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商品详细Cusabio/人白细胞介素18，IL-18 ELISA试剂盒/96T，5×96T，10×96T/CSB-E07450h

Cusabio/人白细胞介素18，IL-18 ELISA试剂盒/96T，5×96T，10×96T/CSB-E07450h

商品编号： CSB-E07450h

品牌： Cusabio

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产地：美国(厂家直采)

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产品分类：夹心法ELISA

公司分类： Sandwich_method_ELISA

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Human Interleukin 18,IL-18 ELISA KIT

Human Interleukin 18,IL-18 ELISA KIT

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Code	CSB-E07450h
Size	96T,5×96T,10×96T
Trial Size
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Product Details

Description	The Human Interleukin 18 (IL-18) ELISA Kit is designed to measure IL-18 biological samples, including serum, plasma, urine, tissue homogenates, in a quantitative fashion. This kit has been verified by multiple tests with advantages of high sensitivity, strong specificity, precision less than 10%, and lot-to-lot consistency. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction as well as colorimetric measurement, the levels of IL-18 in the sample can be calculated. IL-18 is a proinflammatory cytokine belonging to the IL-1 superfamily. It was initially described as an IFN-γ-inducing factor derived from anti-CD3-stimulated T-helper (Th) 1 cell. IL-18 plays a critical role in inflammation and the host defense against microbes. IL-18/IL-18R binding activates adaptor molecule MyD88, which subsequently stimulates the activation of TRAF6 and IRAK1-4, inducing the occurrence of the MAPK and NF-κB pathways that upregulate the expression of various inflammatory cytokines.
Target Name	interleukin 18 (interferon-gamma-inducing factor)
Alternative Names	Iboctadekin ELISA Kit; IFN gamma inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; IGIF ELISA Kit; IL 1 gamma ELISA Kit; IL 18 ELISA Kit; IL 1g ELISA Kit; IL-1 gamma ELISA Kit; IL-18 ELISA Kit; IL1 gamma ELISA Kit; IL18 ELISA Kit; IL18 protein ELISA Kit; IL18_HUMAN ELISA Kit; IL1F4 ELISA Kit; IL1g ELISA Kit; IL1gamma ELISA Kit; ILIF4 ELISA Kit; Interferon gamma inducing factor ELISA Kit; Interferon gamma-inducing factor ELISA Kit; Interleukin 1 gamma ELISA Kit; Interleukin 18 (interferon-gamma-inducing factor) ELISA Kit; Interleukin 18 ELISA Kit; Interleukin-1 gamma ELISA Kit; Interleukin-18 ELISA Kit; Interleukin18 ELISA Kit; MGC12320 ELISA Kit
Abbreviation	IL18
Uniprot No.	Q14116
Species	Homo sapiens (Human)
Sample Types	serum, plasma, urine, tissue homogenates
Detection Range	31.25 pg/mL-2000 pg/mL
Sensitivity	7.8 pg/mL
Assay Time	1-5h
Sample Volume	50-100ul
Detection Wavelength	450 nm
Research Area	Immunology
Assay Principle	quantitative
Measurement	Sandwich
Materials provided	A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human IL-18 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-labeledIL-18 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody. HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution. HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution. Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration. Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances. TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual
Materials not provided	A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Squirt bottle, manifold dispenser, or automated microplate washer. Absorbent paper for blotting the microtiter plate. 100ml and 500ml graduated cylinders. Deionized or distilled water. Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips. Timer Test tubes for dilution.
Troubleshooting and FAQs	ELISA kit FAQs
Storage	Store at 2-8°C. Please refer to protocol.
Lead Time	3-5 working days

Citations

Long noncoding RNA MIAT regulates primary human retinal pericyte pyroptosis by modulating miR-342–3p targeting of CASP1 in diabetic retinopathy X Yu,Experimental Eye Research,2020
The Effects of Protective Ventilation on the Production of Endogenous Melatonin and Prognosis in Patients Undergoing Esophageal Cancer Surgery: A Prospective Randomized DoubleBlind Controlled Study L Wang,Research Square,2020
Ginkgolide B inhibits lung cancer cells promotion via beclin-1-dependent autophagy X Wang,BMC Complementary Medicine and Therapies,2020
Atorvastatin inhibits renal inflammatory response induced by calcium oxalate crystals via inhibiting the activation of TLR4/NF-κB and NLRP3 inflammasome. Sun Y,IUBMB Life,2020
Characterization of Wnt1-inducible Signaling Pathway Protein-1 in Obese Children and Adolescents Wang AR.et al,Current Medical Science,2018
Therapeutic roles of polysaccharides from Dendrobium Officinaleon colitis and its underlying mechanisms Jian Liang.et al,Carbohydrate Polymers,2018
Biomarkers upon discontinuation of renal replacement therapy predict 60╀ay survival and renal recovery in critically ill patients with acute kidney injury Tingting Yang.et al,Hemodialysis International,2017
The relationship between IL-18 and atherosclerotic cardiovascular risk in Egyptian lean women with polycystic ovary syndrome Dawood A.et al,Gynecol Endocrinol,2017
Predictive value of urine interleukin-18 in the evolution and outcome of acute kidney injury in critically ill adult patients Nisula S.et al,Br J Anaesth.,2015
Increased intravitreous interleukin-18 correlated to vascular endothelial growth factor in patients with active proliferative diabetic retinopathy Song Z et al,Graefes Arch Clin Exp Ophthalmol,2014
Correlation of AIM2 expression in peripheral blood mononuclear cells from humans with acute and chronic hepatitis B Wu DL et al,Hum Immunol,2013
The construction of three-dimensional micro-fluidic scaffolds of biodegradable polymers by solvent vapor based bonding of micro-molded layers. Ryu W. et al,Biomaterials,2007

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■Q&A

I have a question about your ELISA Kits for IL18. What are the difference between IL18 binding protein, IL18 receptor 1 and the IL 18 kit? I am working on the earlier stage of renal failure on diabetic patient. So I would like to know which Protein will be more sensitive and efficient for my research.

Thanks for your inquiry.IL18,IL18 binding protein, IL18 receptor 1 are in the IL18 family but they are different proteins.We checked some document related to the renal failure, there is muchresearch on IL18and IL18BP and you can consider this 2 proteins.However, the occurrence of each disease is the result of multi-factor regulation. We suggest you check much more references and choose the proper one.

I amplanning to carry out a study to assess level ofInterleukin from GingivalCrevicular Fluid in Chronic Periodontitis patients before and after treatment.Kindly provide the ELISA kitforanalysis of InterleukinIL- 18. How many sample can be analysed by each kit ?

Thanks for your inquiry.#CSB-E07450h,Human Interleukin 18,IL-18 ELISA KITWe have them all available.They are all appliedwith sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less. If you wan to test GingivalCrevicular Fluid ,we suggest you do pre-testing first.

Target Data

Function	Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Gene References into Functions	The results of the linkage disequilibrium test showed that the -137G/C and -607G/T sites had no linkage disequilibrium in the present study. PMID: 29926984 SNPs associated with genetically determined high activity of TLR5 among patients with CD and genetically determined high IL-12 and IL-18 levels among patients with UC. PMID: 28139755 The adipose tissue IL-18R/IL-18 expression is enhanced in obesity which associates with proinflammatory gene signature and insulin resistance in these individuals. PMID: 28508444 Our study implied that elevated IL-18 expression and downregulation of GAS5 in serums might contribute to insulin resistance in polycystic ovary syndrome patients. PMID: 29648472 Studied serum level of interleukin 18 (IL18) in HIV and Hepatitis C (HCV) coinfection and monoinfection. Found shown that IL-18 is increased in HIV/HCV coinfection when compared with monoinfection and that this increase is likely due to additive sensing of both viruses. PMID: 27927859 not associated with obesity in this study PMID: 28634744 multiple myeloma patients with IL-18 (rs16944) TT had the highest hemoglobin concentration PMID: 30211233 These results suggest that increased IL-18 secretion from larger numbers of neutrophils elicits mechanical hyperalgesia. PMID: 29353540 The results of this study suggests that IL-18 rs187238 and rs1946518 SNPs may be the cause of the AA susceptibility. PMID: 29349811 Studied association of single nucleotide polymorphisms of interleukin-18 (IL 18) and lower extremity deep venous thrombosis in a Chinese population. PMID: 29105174 PI3K is a signal linker between L-selectin and PSGL-1 in IL-18 transcriptional activation at the promoter level. PMID: 29218606 IL-18 is a susceptibility gene for the disease in southern Chinese children. PMID: 30059753 Postoperative serum Cystatin C, IL-18 and Uric acid after 24h were significantly associated with acute kidney injury PMID: 29627485 IL-18 is elevated in polycystic ovary syndrome patients, even in lean ones, and is correlated with insulin resistance and atherosclerotic cardiovascular risk PMID: 29105530 HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690 The IL18 promoter SNP, rs1946518, is a potential risk factor of periodontitis among young Japanese women. PMID: 29129846 The results of this study suggest that IL18 rs187238 and rs1946518 polymorphisms may be associated with an increased risk of gestational diabetes mellitus as well as with BMI in pregnant women. PMID: 28580570 This is the first study to demonstrate that higher pre-treatment IL-18 is associated with HBeAg seroconversion in patients with HIV/HBV co-infection after 48 weeks of HBV cART, which suggests a role for the inflammasome in a favorable response to treatment. PMID: 28195558 Polymorphisms in the IL-18 gene are associated with viral load in Hepatitis B virus . PMID: 30045250 higher level of IL-18 was observed in the monocyte supernatant of the extremely preterm group during late-onset sepsis PMID: 29042061 Increased production of IL-18 in the gut-associated lymphoid tissue and peripheral blood of HIV infected patients. PMID: 29672590 this review discusses IL-18 biological properties and role in disease pathogenesis PMID: 29247988 IL-18 mRNA expression was increased significantly in chronic myeloid leukemia patients PMID: 29097263 these results suggest that the HIV-induced IL-18 plays a role in increased intestinal permeability and microbial translocation observed in HIV-infected individuals. PMID: 29601578 IL-18 gene variants may serve as important biomarkers in predicting the occurrence of nasopharyngeal carcinoma in Asian population. PMID: 29185028 The association of polymorphisms -137 (rs187238) and -607 (rs1946518) in the interleukin-18 (IL-18) promoter with risk of hepatocellular carcinoma (HCC). PMID: 28000712 These findings indicate that the IL-18 rs1946518 SNP in the donor liver is a risk factor for developing bacterial infection after liver transplantation. PMID: 29061223 Serum levels of IL-18 in nephropathic patient significantly rise and Serum levels of IL-18 might be a predictor factor of progression of diabetic nephropathy. PMID: 27663212 A higher IL-18 immunostaining in stroma of IBC and LABC was observed in comparison with tumor cells, but stromal immunoexpression was similar between IBC and LABC. PMID: 28766916 IL-18 was increased in lung myeloid cells in severe chronic obstructive pulmonary disease. PMID: 28830544 a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP.mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668 On PICU day 1, interleukin-18 predicted acute kidney injury with area under the curve=0.82, but neutrophil gelatinase-associated lipocalin and liver fatty acid binding protein predicted acute kidney injury with area under the curve of less than or equal to 0.69; on PICU day 2, area under the curve was higher. Interleukin-18 and liver fatty acid binding protein on day 1 predicted prolonged acute kidney injury. PMID: 28430754 Data show that tumor-derived IL-18 induced PD-1 expression on NK cells and is associated with poor prognosis in triple-negative breast cancer. PMID: 28415798 IL-23, alone and in combination with IL-18 and sRAGE, identified bacterial meningitis with excellent accuracy. Following validation, these markers could aid clinicians in diagnosis of bacterial meningitis and decision-making regarding prolongation of antibiotic therapy PMID: 29394248 cardiac IL-18 and circulating IL-18 are involved in the pathogenesis of Fabry cardiomyopathy and left ventricular hypertrophy. PMID: 27888626 Erdr1 is negatively expressed relative to Il-18 in psoriatic lesional skin. PMID: 26990875 rs187238 was not associated with T1D in the Euro-Brazilian population. PMID: 28973736 IL-18 (mRNA) in skeletal muscle appears to be involved in the regulation of intramuscular lipid metabolism and hypertriglyceridemia PMID: 29342149 The evidence for influenza A virus activation via an indirect, IL-18-dependent mechanism indicates that MAIT cells are protective in influenza, and also possibly in any human disease process in which inflammation and IL-18 production occur. PMID: 27543331 The development of urticaria, asthma, dermatitis, rhinitis, and eosinophilic disorders all have demonstrated correlations to increased IL-18 levels either in the tissue or systemically. IL-18 represents a novel site of immune regulation in not only allergic conditions, but also autoimmune diseases and other instances of aberrant immune functioning. PMID: 27496752 Results suggest that a common functional IL18 haplotype associated with heightened proinflammatory responses confers susceptibility to stress-related depression and anxiety through effects on threat-related amygdala function, a risk pathway specific to women. PMID: 27430614 human platelets contain transcripts for the IL-18 gene. They synthesize the cytokine de novo, process and release it upon activation. PMID: 27914933 abnormal IL-18 expression is induced by genital infection and induces damage to male reproductive capacity, thereby causing male infertility PMID: 26856767 IL-18 level was found to be significantly elevated in CAD patients compared with control individuals PMID: 28059484 The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren"s syndrome. PMID: 28057714 an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120 among systemic sclerosis patients in China, dyspnea was significantly associated with IL-18 -607C/A genotype frequency PMID: 27098064 We conclude that IL-18 protein and messenger RNA levels are increased in the endometrium of patients with PCOS, and this effect is correlated with body mass index. PMID: 27313119 Serum IL-18 levels are significantly elevated in depressive patients. PMID: 27455146 IL-18 cooperates with IL-15 to promote group 3 innate lymphoid cell (ILC3) proliferation and IL-22 production; describe an IL-18-induced, NF-kappaB-mediated mechanism that regulates IL-22 in ILC3s; at steady-state, IL-18 produced by dendritic cells mediates IL-22 production by ILC3s to help maintain normal tissue integrity PMID: 28842466 Show More Hide All
Subcellular Location	Secreted
Protein Families	IL-1 family
Database Links	HGNC: 5986 OMIM: 600953 KEGG: hsa:3606 STRING: 9606.ENSP00000280357 UniGene: Hs.83077

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Human Interleukin 18,IL-18 ELISA KIT

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