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商品详细Cusabio/CD14单克隆抗体/100μl/CSB-MA004879A0m

Cusabio/CD14单克隆抗体/100μl/CSB-MA004879A0m

商品编号： CSB-MA004879A0m

品牌： Cusabio

市场价： ¥7000.00

美元价： 3500.00

产地：美国(厂家直采)

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产品分类：重组抗体

公司分类： Recombinant_antibodies

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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CD14 Monoclonal Antibody

CD14 Monoclonal Antibody

Datasheet

Code	CSB-MA004879A0m
Size	US$350
Image	Western BlotPositive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissueAll lanes: CD14 antibody at 1:2500SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDa Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysateAll lanes: CD14 antibody at 1:1800SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 41 kDaObserved band size: 55 kDa IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of Hela cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of HepG2 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of RAW264.7 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of SY5Y cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Overlay histogram showing Jurkat cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. Overlay histogram showing RAW264.7 cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Target Data

Product Details

Description

This product is the antibody raised in mouse against the human CD14 on the region 20-345aa, which is the mature extracellular portion of the protein in its membrane-bound GPI-anchor form. This monoclonal antibody exists as a non-conjugated isotype IgG1 purified by protein A with a purity greater than 95% and has reactivity against the CD14 from human, mouse, and rabbit. This antibody can be used in ELISA, Western blot (WB), Flow Cytometry (FC), Immunofluorescence (IF), and Immunohistochemistry (IHC), in immunology studies for characterization of disease immune pathways and how the immune system engages in pro-inflammatory processes caused by pathogens. The human monocyte differentiation antigen CD14 participates in the activation of the innate immune response. The CD14 protein is detected in the surface of monocytes and by most tissue macrophages. CD14 regulates immune response against bacteria by recognizing the bacterial LPS and also induces cellular and immune responses against diacylated and triacylated lipopeptides, and electronegative low-density lipoprotein (LDL^-).

Full Product Name

Mouse anti-Homo sapiens (Human) CD14 Monoclonal antibody

Uniprot No.

P08571

Target Names

CD14

Alternative Names

CD 14 antibody; CD_antigen=CD14 antibody; CD14 antibody; CD14 antigen antibody; CD14 molecule antibody; CD14_HUMAN antibody; LPS-R antibody; Mo2 antibody; Monocyte differentiation antigen CD14 antibody; Monocyte differentiation antigen CD14 urinary form antibody; Monocyte differentiation antigen CD14; membrane-bound form antibody; Myeloid cell specific leucine rich glycoprotein antibody; Myeloid cell-specific leucine-rich glycoprotein antibody

Raised in

Mouse

Species Reactivity

Human, Mouse, Rabbit

Immunogen

Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

14G1F3

Purification Method

>95%, Protein A purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA ProtocolWestern Blotting(WB) ProtocolImmunohistochemistry (IHC) ProtocolImmunofluorescence (IF) ProtocolFlow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function	Coreceptor for bacterial lipopolysaccharide
Gene References into Functions	high plasma levels of sCD14 is associated with measles virus infection. PMID: 29795672 a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID: 29959006 CD14 C-(260)T polymorphism is not associated with incidence of acute myocardial infarction in Egyptians who showed elevated serum CD14 levels in PMID: 29874995 This study provides better understanding of the mechanisms and disease susceptibility for MIF and CD14 genetic variants and inflammatory miRNAs networks involved in ankylosing spondylitis and polyarthralgia. PMID: 29863307 Childhood-onset and adult-onset of asthma showed significant difference in allergen sensitivity as well as genetic background with respect to CD14 polymorphism. PMID: 29937881 Renal transplant recipients carrying the CD14-159 TT genotype have significantly higher risk of acute rejection and reduced transplant survival rate than patients with heterozygous or wild-type genotypes. PMID: 28411360 The accumulating evidence does not support an association of CD14 SNP rs2569190 with AR risk. [meta-analysis] PMID: 29687183 It seems that CD14 gene polymorphism might be associated with the risk of CAD, whereas COL4A1 gene polymorphism was not found to confer any risk of CAD PMID: 29299748 higher sCD14 levels in HIV-positive women were associated with a more compromised maternal immunological status and to a lower neonatal birthweight, but not to poorer clinical outcomes in the HIV-exposed children PMID: 29323435 The -221G>C polymorphism of MBL2, the -159C>T polymorphism of CD14 and the TNF-857 polymorphism of TNF-a are risk factors for spinal spinal tuberculosis (TB) and may be involved in the development of spinal TB in the Chinese population. These factors are indicators of susceptibility to spinal TB and require clinical attention. PMID: 29298876 genetic variation of CD14, rs5744455, is related to the susceptibility to laryngeal cancer, providing a theoretical basis for the study of the pathogenesis of laryngeal PMID: 29077168 Calcitriol regulates immune genes CD14 and CD180 to modulate LPS responses in human trophoblasts. PMID: 29089453 The findings suggest that smoking and the presence of TNFalpha-308 GA/AA genotypes may increase the risk for peri-implantitis, while CD14-159 polymorphic CT/TT genotypes decrease the risk. PMID: 28906511 Our results demonstrate that the expression of CD163 and CD206 in monocytes is modulated by LPS in vitro; LPS induces CD163 expression and downregulates the spontaneously increased expression of CD206 PMID: 25914252 may be a simple and easy approach to assess by RT-FCM the reaction between NO and superoxide ion in whole blood monocytes..The no-wash, no-lyse staining protocol with CD45-KO and CD14-PB allows to differentiate clearly and to gate in the monocyte population in near-physiological conditions PMID: 25758468 Association of polymorphic markers of chemokine genes, their receptors, and CD14 gene with coronary atherosclerosis PMID: 29369549 data confirm that engineered human cells expressing TLR4, MD2 and CD14 can respond to CMP with NF-kappaB activation and the response can be influenced by variations in CMP-mannosylation PMID: 29281684 hypothesize that CD14(-159C/T) polymorphic variants might be one of genetic components in the response to attenuated M. bovis BCG bacilli PMID: 29281719 Higher sCD14 levels were found in twins with schizophrenia or bipolar disorder compared to their not affected co-twins, and higher sCD14-levels were associated with psychotic symptoms. PMID: 28039552 novel observation that sCD14 compared with lipopolysaccharide binding protein, offers a preferred target to ameliorate TLR especially TLR4-induced inflammation and insulin resistance in human obesity and metabolic syndrome PMID: 26880233 For asthma, there was suggestive evidence of a gene-by-environment interaction for the CD14 variant rs2569190 (Interaction P = 0.16) but not for the TLR4 variants rs4986790 and rs4986791. PMID: 27977294 the simultaneous detection of both plasma sCD14 and IL-6 is a promising diagnostic approach to identify active pulmonary tuberculosis, and further, measurement of TNF-alpha and sCD163 can identify the most severe cases of tuberculosis. PMID: 27591510 the methylation levels in the promoter region of CD14 gene were higher in the Finnish compared to Russian Karelian children PMID: 27434019 Anti-apoA-1 IgG are independent predictors of nonfatal incident coronary artery disease in the general population. The strength of this association is dependent on a functional polymorphism of the CD14 receptor gene, a finding suggesting a gene-autoantibody interaction for the development of CAD. PMID: 29074586 CD14 haplotypes and sCD14 are important mediators of lung function among those with COPD in this occupationally-exposed population. PMID: 28302109 IL-1beta-primed dendritic cells express high levels of CD14 that mediate IL-17 production through direct interaction with T cells. PMID: 27550748 The distribution of the CD14 rs2569190 genotypes and allele frequencies showed significant differences between the test and control groups. Prevalence of the polymorphic TT genotype and T allele of the CD14 rs2569190 were significantly increased in GCP patients when compared with healthy controls PMID: 28829191 Presepsin is significantly higher in preterm infants with early-onset sepsis compared with uninfected infants. PMID: 27925621 Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14(high)CD16- monocytes independently of systemic inflammatory activity. PMID: 27751505 CXCR7 mediates CD14(+)CD16(+) monocyte transmigration across the blood brain barrier, and is a potential therapeutic target for neuro AIDS. PMID: 28754798 META-ANALYSIS: association between the -159C/T polymorphism in the promoter region of the CD14 gene and sepsis PMID: 28122493 In LPS-stimulated HEK293 cells with low CD14 and high TLR4, no accumulation of PI(4,5)P2 occurred. With an increasing amount of CD14 and decrease of TLR4, 2 peaks of PI(4,5)P2 appeared, approaching those found in LPS-stimulated cells expressing CD14 alone.This suggests that LPS-induced accumulation of PI(4,5)P2 that maximizes TLR4 signaling is controlled by CD14, whereas TLR4 can fine tune this via PI(4,5)P2 turnover. PMID: 27418354 Intermediate CD14++CD16+monocytes might be closely related to the pathogenesis of atrial fibrillation and reflect functional remodelling of the left atrium. PMID: 26826137 In 121 primary sclerosing cholangitis patients, the CD14 -260C>T genotype was associated with development of dominant bile duct strictures (P = 0.02). In 365 liver transplantation patients, TT carriers (4.1%) were protected against the formation of nonanastomotic biliary strictures versus CC/CT patients (12.6%; P = 0.01). PMID: 26970220 response of hPdLSCs to bacterial LPS is strongly augmented by sCD14 PMID: 27504628 analysis of plasma CXCL10, sCD163 and sCD14 in virological suppression and risk of cardiovascular disease PMID: 27355513 TGM2 has a role in macrophage differentiation via mechanisms involving CD14 and SR-AI receptors. PMID: 27378395 we found that higher percentages of circulating CD14+CD204+, CD14+CD163+CD204+ M2-like monocytes were significantly associated with TNM stage, lymph node metastasis, and histological differentiation. PMID: 28639912 these results indicate that CD14 is a co-receptor of TLR4 in the S100A9-induced cytokine response. PMID: 27228163 our data showed the contribution on the TLR4+896A/G and CD14-159C/T polymorphism-related immune dysfunction including increased non-classical (inflammatory) monocyte proportion-related LPS hyper-inflammatory response and decreased classical (phagocytic) monocyte proportion-related impaired phagocytosis in febrile acute de-compensated cirrhotic patients complicated with severe sepsis. PMID: 27861595 Plasma presepsin concentrations are associated with the severity of sepsis and its outcome. PMID: 28099244 Presepsin is a valuable new biomarker for defining severe infections in cirrhosis, proving same efficacy as procalcitonin. However, it is not a useful marker of short-term mortality. PMID: 27895404 Patients with CD14 C (-159) T gene polymorphism, a co-receptor of TLR4, have an increased risk of NAFLD development. PMID: 27895422 Single nucleotide polymorphism CD14-159 C/T was linked to greater Atopic dermatitis risk at 2 to 3 years of age. PMID: 27274543 While there was no association with any respiratory phenotype (as determined by symptoms), the CD14 CT/TT genotype appeared to be protective to increased exposure to NO2 and NO PMID: 27142357 CD14 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267 we report that exogenous CnB is taken up by cells in a time- and concentration-dependent manner via clathrin-dependent receptor-mediated internalization. Our findings further confirm that uptake is mediated by the TLR4/MD2 complex together with the co-receptor CD14 PMID: 27090571 Cord blood sCD14 may be a useful biomarker for predicting infant wheeze and prolonged cough by 1 year of age. PMID: 27144407 Presepsin is a valuable biomarker to detect patients with acute pyelonephritis PMID: 27889429 diagnostic accuracy of presepsin (sCD14-ST) as a biomarker of infection and sepsis PMID: 27823951 Show More Hide All
Subcellular Location	Cell membrane, Lipid-anchor, GPI-anchor, Secreted, Membrane raft, Golgi apparatus
Tissue Specificity	Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Database Links	HGNC: 1628 OMIM: 158120 KEGG: hsa:929 STRING: 9606.ENSP00000304236 UniGene: Hs.163867

品牌介绍

CUSABIO是一家集研发，生产和销售于一体的国家高科技企业。经过12年的努力，CUSABIO已成为全球研究人员的良好合作伙伴。我们致力于为癌症，细胞生物学，免疫学，神经科学，表观遗传学等研究领域的全球客户提供60,000+种经过验证的抗体，7000 +种重组蛋白，603 +种细胞因子和数千种ELISA试剂盒。我们的所有产品都可以满足客户在各个科研领域的不同需求。

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品牌分类

小分子; Molecular Biology Product 活性蛋白质 n. 衣物和装备;成套用品;配套元件; n. 抗体; n. [化]朊，蛋白（质）;

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CD14 Monoclonal Antibody

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